Study on Lactobacillus rhamnosus RAPD System Optimization from Macrobian

Abstract

Abstract: An improved DNA extraction protocol with CTAB was developed for Lactobacillus rhamnosus. DNA was extracted successfully from the samples of 11 strains and was suitable for RAPD analysis. The optimization of a 25μl RAPD was determined as followed: template 50 ng, 2 U TaqDNA polymerase, 3.25 mmol/L dNTPs for each, 5 mmol/L primer, 2.5μl 10×PCR buffer, and 7.5 mmol/L Mg2+. The reaction program was devised for one cycled at 93 ℃ 2 minutes, 36 ℃ 1 minutes, 72 ℃ 2 minutes, which is followed by 40 cycles, each 1 minutes at 93 ℃, 1 minute at 36 ℃, 2 minutes at 72 ℃, and a final extension at 93 ℃ 2 minutes, 36 ℃ 1 minutes, 72 ℃ 10 minutes.

Key words: Lactobacillus rhamnosus, RAPD, optimalization

GU Rui-Xia, CAI Jing-Jing, WANG Qin, YANG Zhen-Quan, REN Yuan-Qing. Study on Lactobacillus rhamnosus RAPD System Optimization from Macrobian[J]. FOOD SCIENCE, 2008, 29(3): 268-271.

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Study on Lactobacillus rhamnosus RAPD System Optimization from Macrobian

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