Assay Taurine in Milk Power for Infant and Young Children with Pre-column Derivatization HPLC

Abstract

Abstract: Objective: Strength the reliability of detemining the assay for taurine in milk power for infant and young children with pre-column derivatization HPLC. Methods: HPLC method was used, chromatography condition included:The instrument was SHIMADZU-10A with a Kromasil C18 column, and the mobile phase was methanol-0.05mol/L NaAc (35:65) at flow rate of 1.0 ml/min. The UV detection wavelenge was 360 nm. Results: Taurine had a good linearity(γ=0.9996)in the range of 0.00～20.34μg/ ml. The relative recovery was 99.6%, RSD was 2.0%(n=5). Conclusion: An accurate method with good reproducibility for control of taurine in milk power for infant and young children were established.

Key words: taurine, assay, HPLC, milk power for infant and young children

HUANG Zhe-Wen, TANG Qin-Qiang. Assay Taurine in Milk Power for Infant and Young Children with Pre-column Derivatization HPLC[J]. FOOD SCIENCE, 2008, 29(3): 415-417.

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Assay Taurine in Milk Power for Infant and Young Children with Pre-column Derivatization HPLC

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