Abstract:  L-glutamine (hereafter referred to as L-Gln) is a acylation-γ-carboxy-glutamic acid, which is in the part of protein structure and is the nitrogen source of biomaterial containing nitrogen. L-Gln plays key role in the cure of gastritis and stomach intestine ulcer, relieving of tiredness, regulating immunity, adjusting cerebral nerves and curing cancer. L-Gln at the market is mainly produced through the fermentation process of microbes. This paper briefly reviewed the advanced development of key fermentation technologies, such as the breeding of producing strain, fermentation processes optimization, separation and purification in down-stream process, especially biosynthetic metabolic engineering, new filtration and coupling technology.

Key words: L-glutamine, fermentation method, breeding of producing strain, metabolic engineering, fermentation process optimization, separation and purification