Study on Extraction Method of Antibacterial Factor from Enterococcus faecalis H1-1 in Koumiss

Abstract

Abstract: The extraction and purification of antibacterial factor from one strain of Enterococcus faecalis H1-1 were studied, which was isolated from koumiss collected from a heardsman’s home in Xilinguole region, Inner Mongolia. The zymotic fluid Enterococcus faecalis H1-1 was precipitated with 50% ammonium sulphate. The sediment was separated with CM-52. The fractions were analyzed for their antibacterial abilities. The antibacterial fraction was isolated with Sephadex G-25 and then G-100 further. The antibacterial fraction isolated with G-100 was analysed with Tricine-SDS-PAGE electrophoresis and three bands were presented. Their molecular weights are 33800 D, 40100 D and 42800 D respectively.

Key words: koumiss, Enterococcus faecali, antibacterial factor, extraction and purification

HE Yin-Feng, DI Guang-Chao, LI Shao-Ying, TIAN Jian-Jun. Study on Extraction Method of Antibacterial Factor from Enterococcus faecalis H1-1 in Koumiss[J]. FOOD SCIENCE, 2008, 29(3): 50-52.

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Study on Extraction Method of Antibacterial Factor from Enterococcus faecalis H1-1 in Koumiss

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