Extraction and Purification of Phycocyanin by the Combined Use of Two-Step Salt Precipitation and Aqueous Two-Phase Extraction from Blue Algae

doi:10.7506/spkx1002-6630-201522002

Abstract

Abstract:

The crude extracts of phycocyanin from fresh blue algal were obtained by freeze-thaw method. The purification of
phycocyanin was performed by the combined use of two-step salting-out precipitation and aqueous two-phase extraction. The
effect of different saturation degrees of (NH4)2SO4 in two-step salt precipitation on purification efficiency was investigated
using the 0.618 method, and the aqueous two-phase system of polyethylene glycol (PEG)-(NH4)2SO4 was established after
comprehensively considering the effect of PEG molecular weight, PEG mass fraction, (NH4)2SO4 mass fraction, pH and
phycocyanin mass concentration on purification efficiency. The results showed that the optimum (NH4)2SO4 saturation was
21% in the first step and 30% in the second step salt precipitation at 25 ℃. Using the aqueous two-phase system containing
10% PEG 1 000 and 25% (NH4)2SO4, the purity of purified phycocyanin was 3.45.

Key words: two-step salting-out precipitation, aqueous two-phase extraction, extraction and purification, phycocyanin

CLC Number:

TQ464.7

ZHANG Fayu, ZHAO Bingbing, CHEN Yu, YUAN Mengyuan, WANG Jiaquan. Extraction and Purification of Phycocyanin by the Combined Use of Two-Step Salt Precipitation and Aqueous Two-Phase Extraction from Blue Algae[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201522002.

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Extraction and Purification of Phycocyanin by the Combined Use of Two-Step Salt Precipitation and Aqueous Two-Phase Extraction from Blue Algae

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