FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (6): 284-287.

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Geometrical Isomer Composition Determination of β,β-carotene from Dunaliella salina

 HUI  Bo-Di, ZHANG  Yan, YE  Shan   

  1. 1.College of Applied Arts and Science, Beijing Union University; 2.College of Life Science, Capital Normal University
  • Online:2008-06-15 Published:2011-08-26

Abstract: In this study, referring to published absorption coefficient (A1% =2500 at 450 nm) of all E-isomer, the absorption coefficients of 9-, and 13-isomers are deduced as 2018 and 1990, respectively, according to the ratio of their spectroscopic and mass responses (eg. peak areas ) on C30-HPLC-PDA-ELSD. According to their chromatographic peak areas, the geometrical isomer composition of β,β-carotene from Dunaliella salina can be actually assessed.

Key words: &beta, ,&beta, -carotene; geometrical isomer; C30 column 1cm;