Abstract: A high-performance liquid chromatographic method was described for the simultaneous determination of saccharine benzoic acid, sorbic acid sodium, and sodium cyclamate in carbonic drink. The effects of operation conditions of HPLC such as the component of the mobile phase, the concentration of ammonium acetate, column and detector temperature, and detector sensitivity setting on determination were investigated. The optimal conditions are as follows: reversed-phase Novo pak-C18 column used for separation with refractive index detector(RID) for detection. Methanol-ammonium acetate (3:97, V/V) as the mobile phase with isocratic elution, column temperature 30 ℃. RID detector sensitivity 64. Under the best conditions, the calibration curve is linear between 2.0μg/ml and 200.0 μg/ml. The correlation coefficients ranges from 0.9995 to 0.9999. The detection limits are 0.2, 0.2, 0.2, 1.3μg/ml respectively. The recoveries of four compositions are 89.0%～104.5%. The relative standard deviations (RSD) are in the range from 2.1% to 3.5%.

Key words: high performance liquid chromatography, refractive index detector, carbonic drink, benzoic acid, sorbic acid, saccharine sodium, sodium cyclamate