FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (7): 235-239.

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Quantitative Detection of Staphylococcus aureus Harboring sea gene in Milk by SYBR Green I Real-time PCR

LI  Yi-Song, WANG  Ming-Na, 吕Qi , ZHANG  Guang-Hui, YAN  Bing, XIANG  Li, JIANG  Yu-Jun   

  1. Key Labortory of Dairy Science, Ministry of Education, College of Food Science and Engineering, Northeast Agricultural University, Harbin 150030, China
  • Online:2008-07-15 Published:2011-07-28

Abstract: In this study, a real-time PCR method was developed to identify Staphylococcus aureus harboring sea gene using a PCR master mix containing DNA-binding dye SYBR Green I. The detection procedure can be achieved for milk samples artificially contaminated by Staphylococcus aureus within 8 h, and the limit of detection is 83 CFU/ml milk.

Key words: Staphylococcus aureus, SYBR Green I, real-time PCR, milk, sea gene