Study on Calcium-enriched Cultivation Condition for Lentinula edodes and Effect of Calcium Ion Concentration on Its Esterase Isozymes

FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (7): 240-242.

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Study on Calcium-enriched Cultivation Condition for Lentinula edodes and Effect of Calcium Ion Concentration on Its Esterase Isozymes

YE Ming, CHEN Hui, YE Chong-Jun

1.College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China; 2.Key Laboratory of Gen and Breed of Mushroom, Agricultural Ministry of China, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China

Online:2008-07-15 Published:2011-07-28

Abstract

Abstract: Three di-mon mating strains (NN22,QS29 and QQ6) of Lentinula edodes were calcium-enriched in PDY medium with Ca2+ concentration range from 0 to 10 mg/ml. The optimization of cultivation conditions of strain NN22 was performed and analyzed by gel electrophoresis of esterase isoenzyme. The results showed that the highest Ca2+ intake of strain NN22 is obtained within 20 days at 25 ℃ and initial pH 7.0 in adding 4 mg/ml Ca2+ PDY. Under the optimized cultivation conditions, Ca2+ content and polysaccharide content of the strain NN22 reach 9.57 mg/g and 3.36 mg/g, respectively. The results also showed that along with the Ca2+ concentration enhancement, the esterase isozymes activities of strain NN22 of Lentinula edodes reduce obviously.

Key words: Lentinula edodes, calcium-enrichment, cultivation condition, esterase isozyme

YE Ming, CHEN Hui, YE Chong-Jun. Study on Calcium-enriched Cultivation Condition for Lentinula edodes and Effect of Calcium Ion Concentration on Its Esterase Isozymes[J]. FOOD SCIENCE, 2008, 29(7): 240-242.

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Study on Calcium-enriched Cultivation Condition for Lentinula edodes and Effect of Calcium Ion Concentration on Its Esterase Isozymes

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