FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (7): 243-246 .

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Study on Enzymological Properties of Linoleic Acid Isomerase from Lactobacillus acidophilus

 WANG  Wu, TANG  Xiao-Ming, FU  Min, PAN  Jian   

  1. 1.School of Biotechnology and Food Engineering, Hefei University of Technology ,Hefei 230009, China; 2.Engineering Research Center of Bio-process, Ministry of Education,Hefei 230009, China
  • Online:2008-07-15 Published:2011-07-28

Abstract: The lionleic acid ismerase from Lactobacillus acidophilus was extracted to study its enzymological properties. The results indicated that the optimum temperature and pH value of lionleic acid ismerase are 40 ℃ and 4 respectively, and the ismerase exhibits strong stability to temperture (20 ~50 ℃) and pH value (4.0~6.0) for 1 h. Various metal ions and their respective concentrations have various effects on activity of the ismerase, and organic solvent as well. The Km of the ismerase is 33.33 mmol/L, and the Vmax is 15.6 mmol/L·h.

Key words: Lactobacillus acidophilus, lionleic acid ismerase, enzymological properties