Solubility, Separation and Purification of Antibacterial Substances Derived from Marine Streptomyces sp. GB-2

Abstract

Abstract: The solubility and purification of antimicrobial substances produced by Streptomyces sp. GB-2 were studied. By determination, it was deduced that the antibacterial substances in GB-2 broth has water solubility property with polarity. They can pass through the ultrafiltration membrane with cut off molecular weight (COMW) 1 kD. By separation on the Sephadex LH-20, two peaks with antibacterial activity appear, in which the first peak displays the strongest inhibition activity against Bacillus cereus and E.coli. In order to further purify the fraction, a high performance liquid chromatography (HPLC) separation was performed. The results showed that the fraction comprises two peaks and the second peak was found to be of antibacterial activity.

Key words: marine Streptomyces GB-2, antibacterial substances, solubility, separation and purification

LU Ying-Jian, DONG Xin, LIU Shu, BIE Xiao-Mei. Solubility, Separation and Purification of Antibacterial Substances Derived from Marine Streptomyces sp. GB-2[J]. FOOD SCIENCE, 2008, 29(7): 306-310 https://doi.org/

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Solubility, Separation and Purification of Antibacterial Substances Derived from Marine Streptomyces sp. GB-2

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