Study on Characteristics of Water Soluble Polysaccharide BPS11 Extracted from Phyllostachys pubescens Leaves by Chromatography

Abstract

Abstract: The polysaccharides from Phyllostachys pubescens leaves were purified by DEAE-52 cellulose column chromatography and Sephadex G-75 glucan gel column chromatography successively,and a kind of polysaccharide named as BPS11 was obtained. In order to study the physicochemical characteristics of the polysaccharide,ultra violet spectroscopy（UV） ,thin-layer chromatography（TLC） and gas chromatography（GC） were employed. Results showed that the components of the polysaccharide include an unknown monosacchride,rhamnose,arabinose,xylose,mannose,glucose and galatose with contents of 0.50%,4.42%,13.90%,6.63%,11.49%,46.06% and 17.00%,respectively. These are remarkably different from data reported in the past literatures.

Key words: Phyllostachys pubescens leaves, water soluble polysaccharide, column chromatography, thin-layer chromatography, gas chromatography

ZHOU Yue-Bin, ZHOU Xiang-Rong, WANG Wei. Study on Characteristics of Water Soluble Polysaccharide BPS11 Extracted from Phyllostachys pubescens Leaves by Chromatography[J]. FOOD SCIENCE, 2008, 29(8): 184-187.

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Study on Characteristics of Water Soluble Polysaccharide BPS11 Extracted from Phyllostachys pubescens Leaves by Chromatography

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