Study on Analysis of Catalytic Reaction Products of Alliin by Alliinase Using HPLC-MS

FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (9): 487-490.

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Study on Analysis of Catalytic Reaction Products of Alliin by Alliinase Using HPLC-MS

CHANG Jun-Min, XIANG Yang, WANG Yan, CHEN Jian

1. College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China;2. Laboratory Department,The First Affiliated Hospital,Xinjiang Medical University,Urumqi 830000,China

Online:2008-09-15 Published:2011-12-08

Abstract

Abstract: Objective: To develop HPLC-MS method for detecting catalytic reaction products of alliin by alliinase. Methods: HPLC-MS equipped with an electro spray ionization (ESI) source was used for this study. Results: The catalytic reaction products of alliin by alliinase are determined as allicin and pyruvic acid. Conclusion: The method is sensitive, simple and accurate, and will provide a basis for the study of allicin in and its pharmacodynamics.

Key words: HPLC-MS, alliin, alliinase, allicin

CHANG Jun-Min, XIANG Yang, WANG Yan, CHEN Jian. Study on Analysis of Catalytic Reaction Products of Alliin by Alliinase Using HPLC-MS[J]. FOOD SCIENCE, 2008, 29(9): 487-490.

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Study on Analysis of Catalytic Reaction Products of Alliin by Alliinase Using HPLC-MS

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