Abstract:

Crude glycolipids extracted from ground fresh carp head with 90% ethanol were subjected to purification by HP- 20 column chromatography and identification by thin layer chromatography (TLC) and infrared (IR) spectrum. And the antitumor activity of the glycolipids was determined by MTT assay for measuring cytotoxicity. The IR spectral analysis showed that the molecules of the analyte contaied the carbohydrate rings with fatty acyl side chain as corresponding acid, which is a general structural feature of glycolipids. Incubation of glycolipids with human breast cancer MCF-7 cells and liver cancer Bel-7402 cells for 24, 48 and 72 h significantly inhibited the growth of caner cells in vitro within the range of 10－400 μg/ml. The HE staining indicated that morphological alterations as indicative of apoptosis were observed in both MCF-7 cells and liver Bel-7402 when incubated with glycolipid extract for 72 h. Theur results suggested that, in breast and liver cancer cells, glycoplipid from carp head delays cell cycle progression by promoting accumulation of cells in S phase without affecting the rate of apoptosis and this S-phase accumulation acts in a significant time-dependent manner.

Key words: carp head, glycolipid, antitumor activity