Abstract:

In order to increase the extraction rate of rapeseed protein, a backward extraction was developed based on reverse micelle system associated with sonication. The backward extraction procedure for rapeseed protein was isooctane recycling, residues dissolving with small amount of KCl buffer solution, appropriate amount of alcohol adding, and then rapeseed protein precipitating in lower aqueous phase. Uniform design was applied to optimize crucial factors for extraction. Optimal condition for backward extraction was achieved through SPSS statistical analysis, which was 2 ml, 1.2 mol/L of K+, 25 ml of KCl buffer solution, and pH 9.5. Extraction rate of rapeseed protein was 59.56% under this optimal backward extraction condition.

Key words: rapeseed protein, backward extraction, uniform design