Abstract:

Alkaloids in red mulberry (Morus rubra L.) were extracted with aqueous ethanol, and quantified using spectrophotometry method and berberine hydrochloride as standard. Effects of ethanol concentration, solid-to-liquid ratio, extraction time and temperature on the alkaloids extraction ratio were studied and optimized by conducting orthogonal test. The optimum extracting conditions were that the red mulberry was extracted with 90% aqueous ethanol, and extracted at 70 ℃ for 6 h with the solid-to-liquid ratio at 1:15. Under such conditions, the extraction yield of alkaloids was 2.5%.

Key words: Morus rubra L., alkaloids, berberine hydrochloride