Abstract:

The aim of this research is to develop a method of CE assay of myoglobin and hemoglobin in animal muscles. The P/ACETM MDQ high performance capillary electrophoresis was applied in the experiment. The electrophoresis buffer system was comprised of 50 mmol/L C6H8O7·H2O and 20 mmol/L Na3C6H5O7·2H2O with pH 2.6. The electrophoresis temperature was 25 ℃ and the voltage was 22 kV. UV detection was monitored at 196 nm. The results showed that the relative standard deviation of myoglobin and hemoglobin in animal muscle was 0.69% and 0.90% and the recoveries of retro-plus standard preparation and pro-plus standard preparation were 93.26% － 97.84%, 90.32% － 97.46% and 79.89% － 84.14%, 79.52% － 83.65%, respectively. This method proved the benefits of convenience, short-time and accuracy and will be a new method for assaying the myoglobin and hemoglobin of muscle flavor substance.

Key words: CE, myoglobin, hemoglobin, muscle