Abstract:

Microwave was used for extraction of chlorophyll from silkworm feces. The extraction conditions were optimized by orthogonal test on the basis of single factor test. The results showed that the optimal extraction conditions are as follows: taking 40% ethanol as extractant, microwave irradiation time 50 s, extraction time 50 min and extraction temperature 70 ℃. Under these conditions, the extraction rate of chlorophyll is 2.684 mg/g, which is increased by 3.4 times compared with traditional organic solvent extraction method. The method has the advantages of high yield, low pollution and low energy consumption.

Key words: microwave-assisted extraction, silkworm feces, chlorophyll, orthogonal test