Construction of Saccharomyces cerevisiae Strain with Barley Lipid Transfer Protein 1 Secretory Capability

doi:10.7506/spkx1002-6630-201001031

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (1): 132-136.doi: 10.7506/spkx1002-6630-201001031

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Construction of Saccharomyces cerevisiae Strain with Barley Lipid Transfer Protein 1 Secretory Capability

ZHANG Hong-bo，RUAN Hui，MA Li-kai，GUO Qin，ZHANG Wei，CHEN Qi-he，
ZHENG Xiao-dong，HE Guo-qing*

(School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China)

Received:2009-04-07 Online:2010-01-01 Published:2014-05-19
Contact: HE Guo-qing* E-mail:gqhe@zju.edu.cn

Abstract

Abstract:

In order to enhance the foam stability of green draft beer, barley lipid transfer protein 1 (LTP1) encoding DNA sequence from Hordeum vulgare was modified to construct yeast Saccharomyces cerevisiae expression plasmid YEp181-KAMLC of barley LPT1 using the plasmid YEplac181. The expression of barley LTP1 was regulated by alcohol dehydrogenase promoter (ADH1 promoter) and terminator cytochrome C terminator (CYC1) of Saccharomyces cerevisiae, and the matured form of barley LTP1 was secreted under the lead of α-mating factor 1 signal peptide. Fermentation for 132 h at 30 ℃ gave a yield of barley LTP1 as high as 29.45 mg/L.

Key words: Saccharomyces cerevisiae, lipid transfer protein 1, beer foam, expression

CLC Number:

TS261.1

ZHANG Hong-bo，RUAN Hui，MA Li-kai，GUO Qin，ZHANG Wei，CHEN Qi-he，. Construction of Saccharomyces cerevisiae Strain with Barley Lipid Transfer Protein 1 Secretory Capability[J]. FOOD SCIENCE, 2010, 31(1): 132-136 https://doi.org/10.7506/spkx1002-6630-201001031

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Construction of Saccharomyces cerevisiae Strain with Barley Lipid Transfer Protein 1 Secretory Capability

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