FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (12): 159-164.doi: 10.7506/spkx1002-6630-201012037

• Analysis & Detection • Previous Articles     Next Articles

Purge and Trap GC-MS for the Identification of Volatile Composition of the Leaves of Different Variants of Perilla L.

HU Yan1,2，DING You-fang1，WEN Chun-xiu3，XIE Xiao-liang3，ZHOU Qiao-mei3，LIU Yu-jun1,*   

  1. 1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China；
    2. Department of Biochemistry, Wenshan University, Wenshan 663000, China；
    3. Medicinal Herbs Research Center, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China
  • Received:2009-10-11 Revised:2010-03-18 Online:2010-06-15 Published:2010-12-29
  • Contact: LIU Yu-jun1,* E-mail:yjliubio@163.com

RichHTML

1

PDF (PC)

582

Abstract:

Purge and trap technique was used for enriching the volatile components of the leaves of different variants of Perilla L. prior to GC-MS analysis. Area normalization method was employed for the subsequent quantification. The results showed that there were significant differences in type and relative contents of volatile components among 4 samples belonging to 3 variants of Perilla L.. Totally 31 volatile components were identified in the samples, 3 of which, namely [Z]-3-hexenal, caryophyllene and santolina triene were contained in all of the samples. The main components of the leaves of wild growing P. frutescens (Linn.) Britt. var. acuta (Thunb.) Kudo included limonene (77.90%), caryophyllene (14.33%), [Z]-3-hexenal (4.9%), and 3-methyl-6-[1-methethyl]-2-cyclohexen-1-one (1.71%), those of the leaves of P. frutescens (Linn.) Britt. var. crispa (Benth.)H. W. Li caryophyllene (30.65%), limonene (24.60%), [Z]-3-hexenal (21.82%) and perilla aldehyde (17.70%), and those of the sample No.1 of P. frutescens (Linn.)Britt. var. frutescen from Anguo, Hebei province 5-ethylidene-1-methyl-cycloheptene (52.79%), [Z]-3-hexenal (19.98%), caryophyllene (13.60%) and perilla aldehyde (9.46%), and those of the sample No.2 of P. frutescens (Linn.)Britt. var. frutescen from Linshan, Guangxi autonomous region perilla aldehyde (31.86%), 5-ethylidene-1-methyl-cycloheptene (31.68%), [Z]-3-hexenal (21.50%) and caryophyllene (10.47%).

Key words: purge and trap technique, GC-MS, Perilla L., volatile substances, relative content

CLC Number: