Determination of 2-Keto-D-gluconic Acid in Fermentation Broth by Polarimetry

doi:10.7506/spkx1002-6630-201022082

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (22): 375-377.doi: 10.7506/spkx1002-6630-201022082

• Analysis & Detection • Previous Articles Next Articles

Determination of 2-Keto-D-gluconic Acid in Fermentation Broth by Polarimetry

SUN Wen-jing1，ZHOU Yan-zheng1，FENG Xiao-yan2，WEI Zhuan3，YU Si-lian4，ZHOU Qiang4

(1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China；
2. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China；
3. Department of Pharmaceutical, Hebei Chemical and Pharmaceutical College, Shijiazhuang 050026, China；
4. Parchn Sodium Isovitamin C Co. Ltd., Dexing 334221, China)

Received:2010-05-07 Online:2010-11-25 Published:2010-12-29
Contact: SUN Wen-jing E-mail:sunwenjing1919@163.com

Abstract

Abstract:

The 2-keto-D-gluconic acid in fermentation broth was calculated on the basis of glucose concentration determined by optical rotation degree. The application feasibility of polarimetry was also investigated. Results indicated that a good linear relationship between optical rotation degree and the concentration of 2-keto-D-gluconic acid and glucose (R2 ＞ 0.9999). The average recovery rate was 102.60% with RSD of 2.17%. This method has characteristics of precision, stability and reproducibility. Therefore, it can be used for determining 2-keto-D-gluconic acid in fermentation broth.

Key words: 2-keto-D-gluconic acid, glucose, fermentation broth, polarimetry, determination

CLC Number:

TQ921.2

SUN Wen-jing1，ZHOU Yan-zheng1，FENG Xiao-yan2，WEI Zhuan3，YU Si-lian4，ZHOU Qiang4. Determination of 2-Keto-D-gluconic Acid in Fermentation Broth by Polarimetry[J]. FOOD SCIENCE, 2010, 31(22): 375-377 https://doi.org/10.7506/spkx1002-6630-201022082

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Determination of 2-Keto-D-gluconic Acid in Fermentation Broth by Polarimetry

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