Abstract:

Objective: To optimize the extraction processing of polyphenols from Malus micromalus Makino. Methods: Based on the single factor experimental analysis, the effects of ethanol concentration, extraction temperature, and extraction time on extraction rate of polyphenols from Malus micromalus Makino were evaluated by response surface methodology. Box-Behnken experimental design was used to explore the optimal extraction processing parameters on the basis of the effects of factors and their cross-interaction on the extraction rate of polyphenols. A regression equation prediction model was established by SAS software for the analysis of response surface values. Results: The optimal extraction conditions of polyphenols from Malus micromalus Makino were ethanol concentration of 52.4%, extraction temperature of 79 ℃ and extraction time of 3.2 h. Under these optimal extraction conditions, the extraction rate of polyphenols was up to 9.25 mg/g. The relative error between the actual extraction rate and predicted extraction rate was 0.22%. Conclusion: The achieved optimal processing conditions suitable for the extraction of polyphenols from Malus micromalus Makino.

Key words: Malus micromalus Makino, polyphenols, extraction, response surface methodology