Detection of Listeria monocytogenes in Food by Sandwich ELISA

doi:10.7506/spkx1002-6630-201024058

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24): 272-276.doi: 10.7506/spkx1002-6630-201024058

• Analysis & Detection • Previous Articles Next Articles

Detection of Listeria monocytogenes in Food by Sandwich ELISA

DUAN Xia1，HUANG Xin2，HUANG Ling-fang1，WEI Hua1，LAI Wei-hua1,*

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China ；
2. Nanchang Xihu District Center for Disease Control and Prevention, Nanchang 330025, China

Received:2010-08-10 Revised:2010-11-15 Online:2010-12-25 Published:2010-12-29
Contact: LAI Wei-hua E-mail:talktolaiwh@163.com

Abstract

Abstract:

A rapid, specific and sensitive sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect L. monocytogenes in food samples, which using polyclonal antibody as the capture antibody and anti-Internalin monoclonal antibody as the detection antibody. Anti-Listeria monocytogenes polyclonal antibody was prepared by using 0.5% formalin inactivated L. monocytogenes as antigen and Japanese rabbits as the host animal. The detection limit was 1.7×105 CFU/mL in pure culture. Results showed that food samples had limited interference to the detection, while pre-enrichment using selective enrichment broth could significantly increase the detection limit and sensitivity.

Key words: Listeria monocytogenes, polyclonal antibody, monoclonal antibody, double-antibody sandwich ELISA

CLC Number:

TS207.3

DUAN Xia1，HUANG Xin2，HUANG Ling-fang1，WEI Hua1，LAI Wei-hua1,*. Detection of Listeria monocytogenes in Food by Sandwich ELISA[J]. FOOD SCIENCE, 2010, 31(24): 272-276 https://doi.org/10.7506/spkx1002-6630-201024058

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Detection of Listeria monocytogenes in Food by Sandwich ELISA

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