Extraction and Saponification of Trans-lutein from Marigold Flowers

doi:10.7506/spkx1002-6630-201024020

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24): 95-101.doi: 10.7506/spkx1002-6630-201024020

• Processing Technology • Previous Articles Next Articles

Extraction and Saponification of Trans-lutein from Marigold Flowers

WANG Chuang1，LI Da-jing1,2,3,*，SONG Jiang-feng2,3，LIU Chun-quan2,3

1. College of Forestry, Northeast Forestry University, Harbin 150040, China；2. Institute of Farm Products Processing, Jiangsu
Academy of Agricultural Sciences, Nanjing 210014, China；3. Engineering Research Center for Agricultural Products Processing,
National Agricultural Science and Technology Innovation Center in East China, Nanjing 210014, China

Received:2010-03-02 Revised:2010-10-26 Online:2010-12-25 Published:2010-12-29
Contact: LI Da-jing E-mail:lidajing@163.com

Abstract

Abstract:

Marigold (Tagetes erecta) flowers were used as raw materials to investigate the extraction and saponification of translutenin from marigold flowers. The effects of organic solvent amount, KOH-ethanol concentration and amount, extraction and saponification temperature and time on extraction and saponification rate of trans-lutenin from marigold flowers was explored. Based on the single factor experimental results, response surface experiments were used to optimize the extraction and saponification efficiency of trans-lutein and a mathematic model between the factors and extraction rate of trans-lutenin from marigold flowers was established. Meanwhile, crude trans-lutein was further purified. Results indicated that the optimal extraction parameters were raw material amount of 1.00 g, petroleum ether amount of 42.6 mL/g, KOH-ethanol concentration of 0.099 g/mL and extraction temperature of 58.8 ℃. Under the optimal extraction conditions, the extraction rate of trans-lutein was up to 1.499%. After the purification of trans-lutein, the content of trans-lutein crystal was up to 90.42%.

Key words: Tagetes erecta, trans-lutein, extraction and saponification, response surface methodology, purification

CLC Number:

TS202.3

WANG Chuang1，LI Da-jing1,2,3,*，SONG Jiang-feng2,3，LIU Chun-quan2,3. Extraction and Saponification of Trans-lutein from Marigold Flowers[J]. FOOD SCIENCE, 2010, 31(24): 95-101.

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Extraction and Saponification of Trans-lutein from Marigold Flowers

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