Abstract:

A simple high-performance liquid homographic (HPLC) method was presented for the determination of 17α- methyltestosterone (17α-MeT) residue in aquatic products. Samples were extracted using ether with the assistance of ultrasounic and cleaned up using petroleum ether. The chromatographic separation was achieved on ODS-C18 column using 77% aqueous methanol solution as an eluant. 17α-MeT was detected using a UV detector and quantified by external standard method. The method revealed spike recoveries for 17α-methyltestosterone in the muscles of T. niloticus, L. vannamei and A. anguilla of not less than 83%, with relative standard deviations of not more than 6.48% and a limit of quantification of 8 μg/kg. The method, thanks to its simplicity and accuracy, is most suitable for the determination of 17α-MeT residue in large quantities of samples.

Key words: 17α-methyltestosterone (17α-MeT), high-performance liquid chromatography (HPLC), optimization, aquatic products