Abstract:

Gallic acid was used as a standard substance to determine total polyphenol contents in different parts of Elaeagnus angustifolia fruit growing in Xinjiang Autonomous Region by Folin-Ciocalteu method. The calibration curve of gallic acid presented good linearity within the concentration range of 2－12 μg/mL (r = 0.9991). The average of three replicates of spike recovery for total polyphenols at three levels was 98.80%, with a relative standard deviation of 1.81%. Elaeagnus angustifolia seeds exhibited significantly higher total polyphenol content than Elaeagnus angustifolia flesh, with a 3- to 5-fold increase. The method demonstrates the advantages of convenience, rapidity and accuracy, thereby providing an effective approach for the quality control of Elaeagnus angustifolia fruit.

Key words: Elaeagnus angustifolia fruit, total polyphenols, Folin-Ciocalteu colorimetry