Separation, Purification and Chemical Characterization of a Polysaccharide Fraction from Polygonatum cyrtonema Hua in Jiuhua Mountain

doi:10.7506/spkx1002-6630-201110011

Abstract

Abstract: The conventional method, enzymolysis, ultrasonic extraction and ultrasonic-assisted enzymolysis were compared for their effectiveness in extracting polysaccharides from Polygonatum cyrtonema Hua in Jiuhua Mountain. Ultrasonic-assisted enzymolysis was evaluated as the best method due to the highest polysaccharide yield. The polysaccharide extract obtained by the selected method was a single polysaccharide component according to DEAE-cellulose column chromatographic analysis. Infrared spectral analysis revealed that the polysaccharide fraction was a hetero polysaccharide. Its relative molecular weight was determined by dextran gel filtration method to be 8912. Based on gas chromatographic analysis, its monosaccharide composition was composed of fructose and glucose at a ratio of 8.7:1.

Key words: polysaccharides from Polygonatum cyrtonema Hua, separation and purification, chemical characterization

CLC Number:

Q539.1

ZHANG Ting-ting，HU Wei，WANG Hao-fen，RAN Ying-xia，ZHENG Chun-yan. Separation, Purification and Chemical Characterization of a Polysaccharide Fraction from Polygonatum cyrtonema Hua in Jiuhua Mountain[J]. FOOD SCIENCE, 2011, 32(10): 48-51.

[1]	XIANG Ting, XIA Chen, LIU Jianhua, WANG Chaoran, SHEN Jianfu. Separation, Structural Identification and Anti-tumor Effects of New Compounds from Cordyceps militaris [J]. FOOD SCIENCE, 2021, 42(8): 235-242.
[2]	LI Yujing, SUN Liping, ZHUANG Yongliang. Purification and Thermal Stability of Geraniin from Rambutan (Nephelium lappaceum) Peels [J]. FOOD SCIENCE, 2021, 42(15): 44-49.
[3]	SHA Yuhuan, MAO Xiaoying, WU Qingzhi, ZHANG Jian, CHENG Weidong. Flavonoid Composition and Antioxidant Activity of Diaphragma juglandis Fructus [J]. FOOD SCIENCE, 2021, 42(12): 91-98.
[4]	CUI Xiaoying, PENG Xinyan, HE Hongjun, ZHANG Min, ZHANG Xiaotong. Separation, Purification and Enzymatic Characterization of Polyphenol Oxidase from Taro [J]. FOOD SCIENCE, 2021, 42(12): 107-115.
[5]	WANG Siqi, HU Yanbo, ZHAI Liyuan, SHI Zenghui, ZHAO Jun. Extraction, Purification and Structure Analysis of Soluble Acidic Polysaccharides from Soybean Dregs [J]. FOOD SCIENCE, 2021, 42(10): 52-57.
[6]	HUANG Qinqin, TIAN Yaping. α-Glucosidase and Dipeptidyl Peptidase IV Inhibitory Activity and Peptide Composition of Porphyra yezoensis Protein Hydrolysate [J]. FOOD SCIENCE, 2020, 41(24): 110-116.
[7]	JIANG Jing, DU Renpeng, GUO Shangxu, ZHAO Dan. Separation, Purification and Biochemical Properties of Exopolysaccharides from Weissella confusa [J]. FOOD SCIENCE, 2020, 41(1): 9-15.
[8]	DENG Ruixue, YANG Xiao, QU Chunxiao, PANG Ye, ZHANG Jianglei, LIU Pu. Chemical Compositions in Different Parts of the Seeds of Three Paeonia Species [J]. FOOD SCIENCE, 2019, 40(8): 141-148.
[9]	HAN Yuzhu, LI Pinglan, HE Qin, ZHAO Zhanzhan, LIU Xuewen, YANG Yemei. Optimization of Extraction Conditions, Separation and Purification of Antifungal Peptides from Hot Pepper Seeds [J]. FOOD SCIENCE, 2019, 40(24): 258-264.
[10]	LU Hongyan, YANG Hang, FANG Li, LIU Shimeng, MIN Weihong. Isolation, Purification and Structural Identification of Antioxidant Peptides Derived from Enzymatic Hydrolysis of Pine Nut Kernel (Pinus koraiensis Sieb. et Zucc.) Albumin [J]. FOOD SCIENCE, 2019, 40(24): 40-45.
[11]	YU Shuqi, BAO Xiaoqing, LIANG Mingtsai, JIN Chenzhong, TIAN Wei. YU Shuqi, BAO Xiaoqing, LIANG Mingtsai, JIN Chenzhong, TIAN Wei [J]. FOOD SCIENCE, 2019, 40(20): 286-292.
[12]	LIU Yaotong, SUN Wei, DONG Mosi, JI Yingxin, LI Tuoping, LI Suhong. Recent Progress in Research on Patatin, the Major Potato Tuber Protein [J]. FOOD SCIENCE, 2019, 40(11): 331-337.
[13]	HE Pingping, WEI Jingliuyi, JIANG Zedong, ZHU Yanbing, NI Hui, LI Qingbiao. Composition and in Vitro Immunostimulatory Activity of Low-Molecular-Mass Fractions of Chemically Degraded Ascophyllan from Ascophyllum nodosum [J]. FOOD SCIENCE, 2019, 40(11): 139-145.
[14]	GAO Zhaojian, XU Xiang, WANG Xianfeng, LU Ning, WANG Xudong, ZHANG Tiezhu, ZHANG Kangzhen, JIAO Wei. Enzymatic Properties and Application in Biomass Conversion of Acidophilic Feruloyl Esterase from Trichoderma atroatroviride [J]. FOOD SCIENCE, 2019, 40(10): 121-128.
[15]	JIN Hongguang, LI Yuquan, LING Yun, ZHANG Lifang, REN Jin, FANG Liang, JIANG Shenhua. Isolation and Structural Identification of Chemical Constituents from Seeds of Raphanus sativus L. [J]. FOOD SCIENCE, 2018, 39(6): 200-206.

Separation, Purification and Chemical Characterization of a Polysaccharide Fraction from Polygonatum cyrtonema Hua in Jiuhua Mountain

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments