Optimization of Process Conditions for Immobilizing Tannase by Corncob

doi:10.7506/spkx1002-6630-201111028

Abstract

Abstract: The process conditions for immobilizing tannase by modified corncob were explored. The Box-Behnken design and response surface methodology were used to optimize immobilization conditions of tannase by modified corncob. The results showed the optimal process conditions were enzyme-to-carrier ratio of 26:1, pH 6.8, immobilization time of 8.0 h and immobilization temperature of 36 ℃. Under the optimal conditions, the activity of immobilized enzyme was (16085 ± 5) U/g, the recovery rate of tannase activity was 44.68%. The optimal reaction pH and temperature of immobilized tannase were 4.5－5 and 60 ℃, respectively.

Key words: immobilized tannase, modified corncob, Box-Behnken design, response surface methodology

CLC Number:

TS201.21

FAN Chao，LI Ji-lie，WANG Hui，HAO Ju-xi，DING Li-xia，LI Zhong-hai. Optimization of Process Conditions for Immobilizing Tannase by Corncob[J]. FOOD SCIENCE, 2011, 32(11): 123-128.

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Optimization of Process Conditions for Immobilizing Tannase by Corncob

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