Optimization of Process Parameters for Enzymatic Preparation of Antihypertensive Peptides from Porcine Hemoglobin

Abstract

Abstract: In order to prepare antihypertensive peptide with high activity, trypsin was selected to hydrolyze porcine hemoglobin. The one-factor-at-a-time and orthogonal array design methods were used to optimize hydrolysis conditions such as substrate concentration, temperature, pH, enzyme dosage and time based on the ACE inhibitory rate of hydrolyzed porcine hemoglobin. The results showed that the optimal hydrolysis conditions were hydrolysis for 8 h at 45 ℃, pH 8.0, an enzyme dosage of 2000 U/g and a substrate concentration of 10 g/100 mL. Under these hydrolysis conditions, the ACE inhibitory rate of porcine hemoglobin hydrolysate was 68.74%.

Key words: antihypertensive peptide, porcine hemoglobin, trypsin, enzymatic hydrolysis process

CLC Number:

TS201.2

References

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