Effect of Inducers on Xylanase Activity and Biosynthesis of Ferulic Oligosaccharides in Aureobasidium pullulans

Abstract

Abstract: Feruloyl oligosaccharides (FOs) could be produced as a result of the hydrolysis of wheat bran fiber by xylanase synthesized during fermentation by a mutant strain of Aureobasidium pullulans without the secretion of melanin bred using wheat bran. The effects of inducers such as carbon source, nitrogen source, metal ion and surfactant on the activity of xylanase and the formation of FOs were investigated. The medium composition for FOs production by the strain was optimized using the orthogonal array design method. The highest Fos production and xylanase activity, 627 nmol/L and 52.66 IU/mL, respectively, were achieved by using a medium comprised of 50 g/L wheat bran, 15 g/L glucose, 1 g/L peptone and 1 g/L corn syrup.

Key words: wheat bran, ferulic oligosaccharides, inducer, biosynthesis and control, Aureobasidium pullulans

CLC Number:

Q939.97

Xiao Hong-Yu. Effect of Inducers on Xylanase Activity and Biosynthesis of Ferulic Oligosaccharides in Aureobasidium pullulans[J]. FOOD SCIENCE, 2012, 33(1): 209-214.

References

[1] Luc S., Jacqueline V., Jean-Francois T., Isolation and partial characterization of feruloylated oligosaccharides from maize bran, Carbohydr Res. 1995, 272: 241-253
[2] Wang J., Yuan X. P., Sun B. G., Cao Y P., Tian Y., Wang C. T., On-line separation and structural characterisation of feruloylated oligosaccharides from wheat bran using HPLC-ESI-MSn [J]. Food Chem. 2009, 115:1529–1541
[3] Yuan X P., Wang J., Yao H. Y., Production of feruloyl oligosaccharides from wheat bran insoluble dietary fibre by xylanases from Bacillus subtilis [J]. Food Chem. 2006, 95: 484–492
[4] Ella A., John R., Hans S., Mirko B., Isolation and structural identification of complex feruloylated heteroxylan side-chains from maize bran [J]. Phytochemistry. 2006, 67:1276–1286
[5] Ferreira P., Diez N., Faulds C.B., Soliveri J., Copa-Pati?o J.L.. Release of ferulic acid and feruloylated oligosaccharides from sugar beet pulp by Streptomyces tendae [J]. Bioresource Technol. 2007, 98: 1522–1528
[6]Moreau A,Durand S,Morosoli R.Secretion of a Crypto coccus albidus xylanase in Saccharomyces cerevisiae[J] Gene,1992,116(1):109-13.
[7]Chun-yanXie,Zhen-xinGu*,XuejiaoYou.Screening of edible mushrooms for release of ferulic acid from wheat bran by fermentation[J]. Enzyme and Microbial Technology,2010,46: 125–128.
[8]Kim HO, Lim JM, Joo JH, Kim SW , Hwang HJ, Choi JW, Yun JW. Optimization of submerged culture condition for the production of mycelial biomass and exopolysaccharides by Agrocybe cylindracea [J]. Bioresource Technology, 2005, 96:1175-1182
[9]Hwang HJ, Kim SW, Xu CP, Choi JW, Yun JW, Production and molecular characteristics of four groups of exopolysaccharides from submerge culture of Phellinus gilvus [J]. Journal Applied Microbiology, 2003, 94: 708-719.
[10] Liu LH, Lin ZW, Zheng T, Lin L, Zheng CQ, Lin ZX, Wang SH, Wang ZH, Fermentation optimization and characterization of the laccase from Pleurotus ostreatus strain 10969 [J]. Enzyme and Microbial Technology, 2009, 44:426-433.
[11] Garraway, MO, Evans RC, Fungal Nutrition and Physiology. John Wiley, New York.1984.
[12] Lee BC, Bae JT, Pyo HB, Choe TB, Kim SW, Hwang HJ, Yun JW. Submerged culture conditions for the production of mycelial biomass and exopolysaccharides by the edible Basidiomycete Grifola frondosa [J]. Enzyme and Microial Technology, 2004, 35:369-376.
[13] Petrescu I,Lamotte-Brasseur J,Chessa J P,Ntarima P Claeyssens M,Devreese B,Marino G,Gerday C.Xylanase from the psychrophilic yeast Cryptococcus adeliae[J] Extremophiles,2000,(4):137-144.
[14]Amoresano A,Andolfo A,Corsaro M M,et al.Structura characterization of a xylanase from psychrophilic yeast by mass spectrometry.Glycobiol,2000,10(5):451-458.
[15] 付文杰，张莎莎. 茶薪菇液体发酵培养基优化研究[J]. 安徽农业科学, 2007, 35: 5668-5669
[16] Oliveira L. A., Porto A. L. F., Tambourgi E. B., Production of xylanase and protease by Penicillium janthinellum CRC 87M-115 from different agricultural wastes [J]. Bioresource Technol., 2006, 97: 862–867
[17] Cai Q. H., Yue X. Y., Niu T. G., Ji C., Ma Q. G., The screening of culture condition and properties of xylanase by white-rot fungus Pleurotus ostreatus [J]. Process Biochem., 2004, 39: 1561–1566
[18] ZHANG Jian-zhen,PANG Zong-wen,LIANG Jing-juan. The Biosynthesis of Xylanase by Aureobasidium pulluans XYE-7[J]. Modern Food Science and Technology,2007,23(1):5-7.
[19] 孙东平. 灵芝深层培养基及灵芝多糖纯化的研究[D]. 华南理工大学, 1996.
[20] Xu GQ, Chu J, Zhuang YP, Wang YH, Zhang SL. Effects of vitamins on the lactic acid biosynthesis of Lactobacillus paracasei NERCB 0401 [J]. Biochemical Engineering Journal, 2008, 38:189-197
[21] Li J, Li P, Liu F. Production of theanine by Xerocomus badius (mushroom) using submerged fermentation [J]. LWT, 2008, 41:883-889.
[22] Zeng GM, Shi JG, Yuan XZ, Liu J, Zhang ZB, Huang GH, Li JB, Xi BD, Liu HL. Effects of Tween 80 and rhamnolipid on the extracellular enzymes of Penicillium simplicissimum isolated from compost [J]. Enzyme and Microbial Technology, 2006, 39: 1451-1456
[23] Park JP, Kim SW, Hwang HJ, Cho YJ, Yun JW. Stimulatory effect of plant oils and fatty acids on the exo-biopolymer production in Cordyceps militaris [J]. Enzyme and Microbial Technology, 2002, 31:250-255
[24] Hsieh Cy, Wang HL, Chen CC, Hsu TH, Tseng MH. Effect of plant oil and surfactant on the production of mycelial biomass and polysaccharides in submerged culture of Grifola frondosa [J]. Biochemical Engineering Journal, 2008, 38:198-205