Abstract: Lactobacillus, an important kind of physiological bacteria in the human body, plays an important role in maintaining human intestinal ecosystem environment and intestinal health. However, the most important premise for lactobacillus to execute its physiological functions is the adhesion on the surface of intestinal cells. More and more studies have showed that S-layer protein has the ability to adhere to intestinal cells. For example, Lactobacillus reuteri can adhere to mucoprotein and HT-9 cells. Various methods such as SDS-PAGE, chromatographic purification, and microscopic observation have been successfully applied to study the adhesion of Lactobacillus to intestinal cells. The applications of surface plasma resonance and immunological techniques to study S-layer protein adhesion have gained extensive attention as a result of the development of new techniques. In this paper, recent research advances in Lactobacillus S-layer proteins and their adhesion are reviewed.

Key words: Lactobacillus, S-layer protein, adhesion, extraction, purification