Determination of EPA, DPA and DHA in Seal Oil by Gas Chromatography

Abstract

Abstract: Objective: To establish a method for determining ω-3 polyunsaturated fatty acids including eicosapentaenoic acid (EPA), docosapentenoic acid (DPA) and docosahexaenoic acid (DHA) in seal oil. Methods: Fatty acids in seal oil were methyl esterified using a KOH-methanol solution. The applicability of the developed method was validated. Results: This method revealed an excellent liner relationship for methylated EPA, DPA and DHA in the concentration range of 10－2000μg/mL with recovery rate of 96.93%－103.38% and RSD less than 2%. Conclusion: This method is simple, rapid, accurate and suitable for the analysis of fatty acids in seal oil.

Key words: seal oil, eicosapentaenoic acid (EPA), docosapentenoic acid (DPA), docosahexaenoic acid (DHA), content determination

CLC Number:

TS207.3

References

[1]孙焕，张春红，黄晓杰.酶改性大豆分离蛋白作为壁材微胶囊化海豹油研究[J].粮食与油脂，2006，5：3-5.
[2]Julie A.Conquer, Lynn A. Cheryk, Ed Chan, et al. Effect of Supplementation with Dietary Seal Oil on Selected Cardiovascular Risk Factors and Hemostatic Variables in Healthy Male Subjects [J]. Thrombosis Research, 1999,96( 3): 239-250.
[3]Gulen Arslan, Linn Anne Brunborg, Livar Fr?yland,et al. Effects of duodenal seal oil administration in patients with inflammatory bowel disease[J].Chemistry and Materials Science, 2002, 37(10): 935-940.
[4]Mary G. Murphy, Valerie Wright, Robert G. Ackman, et al. Diets enriched in menhaden fish oil, seal oil, or shark liver oil have distinct effects on the lipid and fatty-acid composition of guinea pig heart. Biomedical and Life Sciences, 1997,177 (1):257-269.
[5]肖玫，欧志强.深海鱼油中两种脂肪酸(EPA和DHA)的生理功效及机理的研究进展[J].食品科学，2005,26（8）：522-524.
[6]Robert C. Block, William S. Harris, Kimberly J. Reid,et al. EPA and DHA in blood cell membranes from acute coronary syndrome patients and controls[J]. Atherosclerosis,2008,197(2): 821-828.
Penny M. Kris-Etherton, William S. Harris,Lawrence J. Omega-3 Fatty Acids and Cardiovascular Disease New Recommendations From the American Heart Association[J]. Arteriosclerosis, Thrombosis, and Vascular Biology. 2003;23:151-152.
[8]Eliz Anil. The impact of EPA and DHA on blood lipids and lipoprotein metabolism: influence of E genotype[J]. Proceedings of the Nutrition Society, 2007, 66: 60-68.
[9] 陈亚飞程速远孙宇.毛细管气相色谱法测定海狗油胶丸中ω-3多不饱和脂肪酸的含量[J].药物分析杂志,2004，24(5):499-501.
[10]Hirotsugu Ueshima; Jeremiah Stamler; Paul Elliott, et al. Food Omega-3 Fatty Acid Intake of Individuals (Total, Linolenic Acid, Long-Chain) and Their Blood Pressure[J]. Hypertension,2007,50:313.
[11]Philip C. CALDER. n-3 Fatty acids and cardiovascular disease: evidence explained and mechanisms explored[J]. Clinical Science ,2004, 107:1–11.
[12]广东省食品卫生监督检验所.GB/T 5009.168-2003 食品中二十二碳五烯酸和二十二碳六烯酸的测定[S].北京：中华人民共和国卫生部中国国家标准化管理委员会，2003:391.
[13]刘爱琴，罗超杰，孙晓霞. 气相色谱法测定鱼油微胶囊中EPA和DHA的含量[J].中国食品添加剂，2010,4:273-275.
[14]全文琴，陈小娥，陈洁. 高效液相色谱/质谱联用直接测定鱼油中EPA /DHA含量[J].食品与机械，2008,24（2）:114-116.
[15]张秋会,柳艳霞,赵改名. 8种海洋微藻中EPA和DHA含量的测定[J].食品与机械，2006,22（6）:113-114.
[16]国家药典委员会.中国药典二部[M].2010版.北京：中国医药科技出版社，2010：附录194.
[17]周敏,陶宁萍,王锡昌.黄鳍金枪鱼眼窝油中DHA含量测定方法的优化[J].湖南农业科学,2010，(11)：93-95.
[18]范亚苇,邓泽元,余永红．不同脂肪酸甲酯化方法对共轭亚油酸分析的影响[J]．中国油脂，2007，32(1)：52-55．
[19]李庆民,陈桂范,高实.气相色谱外标法测定鱼油中EPA和DHA的含量[J].沈阳药科大学学报,1996,13(4):259-261.

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