FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (14): 219-222.

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Comparative Determination of Fatty Acids in Pine Pollen from Different Varieties by GC-MS

Wei CHEN,XIU-xia SUN,WEI-wei LIU,XU-ran WU,YONG-ming LIU   

  • Received:2011-06-08 Revised:2012-05-29 Online:2012-07-25 Published:2012-07-27
  • Contact: XU-ran WU E-mail:ytdxwxr@126.com

Abstract: A GC-MS method for the determination of fatty acids in pine pollen was established using Soxhlet extraction, methyl esterification and GC-MS and applied to comparatively analyze the relative contents of 12 main fatty acids in pone pollen from different varieties. This method provided a rapid, effective strategy for the determination of fatty acids in pine pollen. Except palmitic acid and heptadecanoic acid, all other 10 identified fatty acids showed a significant difference in their relative average contents (P <0.05). Moreover, both the relative average contents of saturated and unsaturated fatty acids exhibited a highly significant difference among five investigated varieties of pine pollens (P < 0.01).

Key words: pine pollen, fatty acid, GC-MS

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