Optimization of Microwave Extraction of Polysaccharides from Flammulina velutipes Scraps by Response Surface Methodology

Abstract

Abstract: Microwave assistance was used for extracting polysaccharides from Flammulina velutipes scraps. The extraction process was optimized using response surface methodology. The results of one-factor-at-a-time experiments and Box-Behnken design showed that extraction time, solvent-to-solid ratio and microwave power had a significant effect on extraction efficiency. The optimized extraction conditions were extraction time of 85 s, solvent-to-solid ratio of 23:1 (mL/g), microwave power of 320 W. Under these conditions, the extraction rate of polysaccharides was 13.12%.

Key words: Flammulina velutipes, polysaccharides, microwave extraction, response surface methodology

CLC Number:

TS201

References

[1] ZHENG Yi, Li Chao, WANG Nai-xin. Research progress in polysaccharides from Flammulina velutipes [J]. Food Science, 2010, 31(17):425-428.
[2] KONG Xiao-xue, AN Xin-xin, ZHAO Li-yan,et al.Optimization of extraction processing for water-soluble polysaccharides from Flammulina velutipes [J]. Food Science, 2010, 31(24):230-235.
[3] TIE Mei, LI Chuang, FEI Jin-yan, et al. Study on the purification and IR spectroscopy of Se-polysaccharides in selenium-enriched Flammulina velutipes [J]. Journal of Instrumental Analysis, 2006, 27(2):158-161.
[4] Zou Yu-xiao,Liao Sen-tai,Wu Yu-ming et al. The effects of the Flammulina velutipes polysaccharide extract on learning and memory ability of scopolamine hydrobromid induced model mice and rats. Journal of Chinese Institute of Food Science and Technology, 2010, 1:26-230.
[5]Li Yi-fang, Zeng Huai-wei, Wang Min, et al. The protective effect of polysaccharides of Flammulina velutipes on acute liver injury in mice. Journal of Guangdong pharmaceutical college. 2010, 26(2):162-165.
[6] Zhang Jian, Yao Wen-bin,Xie Chen,et al. Studies on active principle of Flammulina velutipes polysaccharides containing germanium with hepatoprotective effect [J].Chinese journal of biochemical phamaceutics, 2007, 28(6): 366-368.
[7] Leung M Y K, Fung K P. The isolation and characterization of an immunomodulatory and anti-tumor polysaccharide preparation from Flammulina velutipes [J]．Immunopharma-cology，1997，35：255-263．
[8] Fhemanda R. Smiderle, Elaine R. Carbonero, Guilheme L. Sassaki. Characterization of a heterogalactan: Some nutritional values fo the edible mushroom Flammulina velutipes [J]. Food Chemisty, 2008, 108:329-333.
[9] LIU Xiao-peng, JIANG Ning, XIANG Dong-shan, et al. Study on submerged fermentation conditions of Flammulina velutipes and extraction of mycelial polysaccharides by water Bath[J]. Food Science, 2008, 29(9):437-440.
[10] Huang Pu, Xi Ming-yong, Nie Shao-ping, et al. Study on microwave-assisted extraction of polyxaccharides from spore of Ganoderma atrum with response surface analysis [J]. Food Science, 2007, 28(10):200-203.
[11] SHI De-fang, GAO Hong, TAN Hong-zhuo, et al. Study on microwave-assisted extraction and biological activity of polysaccharide from Lentinus Edodes stem [J]. Food research and Development, 2010, 31(2):10-14.
[12] ZHANG Yan-rong, SHAN Yu-ling, LIU Ting-ting, et al. Application of microwave extraction in extracting Agarucysbkazeu polysaccharides [J]. Food Science, 2006, 27(12):267-270.
[13] LI Lei, ZHANG Jin-ping, ZHOU Quan-cheng. Optimization of microwave-assisted extraction of polysaccharides in the flower of Platycodon grandiflorum by response surface methodology [J]. International agriculture and biological engineering, 2009,2(2): 65-74.
[14] Hou Xue-jie, Chen Wei. Optimization of extraction process of crude polysaccharides from wild edible BaChu mushroom by response surface methodology [J]. Carbohydrate Polymers, 2008,72:67-74.
[15] RUAN Zheng, ZHOU Quan-cheng, DENG Ze-yuan, et al. Study on optimization of extraction conditions of Platycodon grandiflorum A. DC polysaccharide using response surface methology [J]. Food Science, 2008, 29(12):283-287.