Medium Optimization for Antifungal Substance Production by Brevibacillus brevis FM4B Using Response Surface Methodology

Abstract

Abstract: Plackett-Burman design was used to evaluate the effects of 9 medium components on the production of antifungal substance by Brevibacillus brevis FM4B. The results showed that glucose, peptone and K2HPO4 were the most important medium components. Steepest ascent procedure and response surface methodology based on Box-Behnken experimental design were employed to optimize the three medium components. The optimal fermentation medium for producing antifungal substance was composed of glucose 6.1 g/L, peptone 23.1 g/L, sucrose 31.3 g/L, K2HPO4 0.825 g/L and MgSO4·7H2O 0.5 g/L. The inhibition spot area of the optimized culture medium after fermentation with Brevibacillus brevis FM4B was increased by 42% compared to that of the original medium.

Key words: Brevibacillus brevis, biological control, central composite design, response surface methodology, medium optimization

CLC Number:

中图分类号：S432.4

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