Determination of Difloxacin Hydrochloride Residue in Animal Liver by Solid Phase Extraction and Flow Injection Chemiluminescence

Abstract

Abstract: A new quick method for the determination of difloxacin hydrochloride residue in pork and chicken livers using solid phase extraction followed by flow injection chemiluminescence analysis was presented based on the inhibition of chemiluminescence generation from the reaction between luminol and hydrogen peroxide by difloxacin hydrochloride. The results showed that an excellent linear relationship between chemiluminescence intensity and negative logarithm of difloxacin hydrochloride concentration was observed in the range of 1.00 × 10-5－1.00×10-9 mg/mL. The limit of detection for difloxacin hydrochloride was 4.07 × 10-9 mg/mL. The precision was 0.93% (relative standard deviation, RSD) for eleven replicate determinations. Therefore, this method can be used for the determination of difloxacin hydrochloride in animal liver with satisfactory results.

Key words: difloxacin hydrochloride, flow injection chemiluminescence, animal liver, solid phase extraction

CLC Number:

O657.3
R917

ＧＡＯ xiang-yang. Determination of Difloxacin Hydrochloride Residue in Animal Liver by Solid Phase Extraction and Flow Injection Chemiluminescence[J]. FOOD SCIENCE, 2012, 33(16): 184-187.

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