Optimization of Extraction Process for Limonoids from Navel Orange Residues by Response Surface Methodology

Abstract

Abstract: An ultrasonic-assisted method to extract limonoids from navel orange residues left over from juicing using ethanol as the extraction solvent was proposed and optimized. Ethanol concentration, ultrasonic treatment time and extraction temperature were identified as main parameters that influence limonoids yield using Plackett-Burman design combined with the steepest ascent method. A regression equation reflecting the relationship between limonoids yield and these main affecting parameters was established based on Box-Behnken experimental design to optimize the three extraction parameters using response surface methodology. The optimal ethanol concentration, ultrasonic treatment time and extraction temperature were 82%, 18 min and 60 ℃, respectively. Under these conditions, the actual extraction yield of limonoids was 1.632‰, which was close to the predicted value of 1.651‰.

Key words: navel orange residue, limonoids, extraction, response surface methodology, optimization

CLC Number:

TS201.2

References

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