Prediction of Cathepsin B and L Activities in Cured Silver Carp during Drying Using Response Surface Methodology

Abstract

Abstract: The activities of cathepsin B and L in cured silver carp (Hypophthalmichthys molitrix) were determined during curing and heat pump drying. Three process parameters (temperature, pH and salt concentration) were identified as main variables that influence the activities of cathepsin B and L. According to the properties of both enzymes and the results of physiochemical analysis of cured silver carp during drying, different levels of the main variables were selected to study their effects on the activities of cathepsin B and L by response surface methodology. A separate predicative regression model for the activities of both cathepsin enzymes was proposed and applied. The results indicated that the proposed regression models could be used to predict the activities of cathepsin B and L. The activities of both enzymes in cured silver carp showed a downward trend during processing and 43.18% and 26.08% of their original activities were retained at the end of the drying stage, respectively. The actual activity of cathepsin B before curing, after curing, and after drying for 2, 5 d and 7 d was 36.7%, 39.9%, 46.5%, 46.5% and 53.5% of its potential activity, respectively; the actual activity of cathepsin L at these time points was 6.6%, 5.6%, 10.4%, 10% and 12.6% of its potential activity, respectively as demonstrated by model predictions.

Key words: cured silver carp (Hypophthalmichthys molitrix) , heat pump drying, cathepsin B,L, response surface methodology, prediction model

CLC Number:

S377

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