Effect of Juglone on the Cell Membrane of Escherichia coli

Abstract

Abstract: Juglone (5-hydroxyl-1,4-naphthalene hydroquinone), a natural antibacterial compound, has reportedly showed antibacterial effect on many microbial species. However, its antibacterial mechanism has never been reported. In this study, the effect of juglone on the cell membrane of Escherichia coli during culture in the presence of juglone at various concentrations was evaluated in terms of relative conductivity, protein content, K+ and Na+ efflux, and also examined under transmission electron microscopy (TEM). The results showed that juglone increased membrane permeability and therefore resulted in an outflow of charged ions and protein. Compared with the control samples, K+ and Na+ concentrations revealed a significant change. TEM observation exhibited that E. coli cells were destroyed by juglone, causing thin cell membrane, blurred cell boundary and outflowed cytoplasm. In addition, increasing juglone concentration could result in more obvious damage to the cell membrane in E. coli. In conclusion, the inhibitory activity of juglone is directly related to its damage to the cell membrane of E. coli.

Key words: juglone, Escherichia coli, cell membrane, effect

CLC Number:

TS201.3

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