Effect of Intense Pulsed Light Irradiation on Membrane Damage and Intracellular Enzyme Activities in Aspergillus niger

Abstract

Abstract: This study was designed to investigate changes in the membrane permeability and intracellular enzyme activities of Aspergillus niger before and after intense pulsed light irradiation. The results indicated that protein spillage and MDA leakage in Aspergillus niger suspension after intense pulsed light irradiation revealed an obvious increase when compared with the control group. Meanwhile, uneven and wrinkle cell membranes and walls were observed by transmission electron microscopy. Moreover, the activities of dehydrogenase and other major intracellular antioxidant enzymes also revealed a decreasing trend. Therefore, the destruction of the barrier structure of mold could result in changes in the osmotic pressure and cell membrane permeability, thus leading to changes to intracellular substances, and even cell death.

Key words: intense pulsed light, Aspergillus niger, membrane damage, intracellular enzyme activity

CLC Number:

TS205.9
Q689

References

[1] 陆蒸.食品冷杀菌技术-脉冲强光杀菌[J]. 浙江农村机电, 2005，(2):17-17.
[2] 罗志刚,杨连生.脉冲强光技术在食品工业中的应用[J].食品工业, 2002，(5):44-46.
[3] S.J. MacGregor,N.J. Rowan,L. McIlvaney,J.G. Anderson,R.A. Fouracre,& O. Farish．Light inactivation of food related pathogenic bacteria using a pulsed power source[J]．Letters in applied microbiology，1998，27(2)：67-70．
[4] N J Rowan,S J MacGregor,J G Anderson,R A Fouracre,L McIlvaney,O Farish．Pulsed light inactivation of food related microorganisms[J]．Applied and environmental microbiology，1999，65(3):1312-1315．
[5] Takeshita K，Yamanaka H，Snmeshima T，et a1．Sterilization effect of pulsed light on various microorganisms[J]．Journal of Antibacterial and Antifungal Agents(Japan),2002,30(5)：277-284．
[6] 江天宝，曹玉兰，陆蒸等．脉冲强光对烤鳗的杀菌效果及感官品质的影响[J]．农业工程学报，2006，22(12)：200-204．
[7] Soojin Jun,Joseph Irudayaraj *,Ali Demirci,& David Geiser．Pulsed UV-light treatment of corn meal for inactivation of Aspergillus niger spores[J]．International Journal of Food Science & Technology，2003，38(8)：883-888．
[8] R.R. Sharma, A.Demirci.Inactivation of Escherichia coli O157:H7 on Inoculated Alfalfa Seeds with Pulsed Ultraviolet Light and Response Surface Modeling[J].Journal of food science,2003,68(4):1448-1453
[9] Kathiravan Krishnamurthy,All Oemirc,Joseph M Irudayaraj．Milk Pasteurization by Pulsed UV Iight Treatment．Paper number 046048．2004 ASAE Annual Meeting．
[10]Takeshita K，Shibato J．SameshimaT，et a1．Damage of yeast cells induced by pulsed
1ight irradiation[J]．International Journal of FoodMicrobiology.2003,85(2):151-158．
[11] AIex Wekhof．Pulsed UV to Sterilize Food Packaging and to Preserve Food Stuffs[J]．News letter of International UV Association(Canada),2002,4(5):4-8．
[12] 周万龙，高大维，夏小舒．脉冲强光杀菌技术的研究[J]．食品科学，1998,19(1)：16-19．
[13]马凤鸣，张佰清，徐江宁等．脉冲强光杀菌装置设计的初步研究[J]．食品与机械，2005，21(6)：66-67．
[14] 李静，廖小军，钟葵等.高压脉冲电场对酿酒酵母钝化机理研究[J].农业工程学报，2011,27（4）：355-360.
[15]陈雅蕙，袁明秀．生物化学实验原理和方法[M]．北京：北京大学出版社，2006:240-242.
[16]刘彬，齐云，李蒙等.分光光度法与荧光法测定肝组织丙二醛含量的比较研究[J]. 中国药理学通报,2010,26(12):1674-1677.
[17]杨天佑，田静，黄新等. TTC-脱氢酶测定法表征低温真空环境对细菌呼吸活性的影响[J].食品与机械，2008（6）：32-35.
[18]董海胜，陈斌.利用修改的Marklund方法测定SOD活性[J].保鲜与加工,2009, 9(1):27-29.
[19]胡斌，曾庆梅．超高压对枯草芽抱杆菌中三种抗氧化酶活性的影响[D].合肥：合肥工业大学，2007.
[20]蒙秋锁，黄青．应用DTNB直接显色法测定全血谷胱甘肽氧化酶活力的研究．陕西医学检验,1994,9(3):169-170.