Investigation of Salmonella Contamination in Broiler Slaughter and Processing Chain and ERIC-PCR Analysis of Its Sources

Abstract

Abstract: Objective: In the present study, the contamination of Salmonella in the slaughter and processing chain of broilers was investigated and the sources were traced with the aim of determining the critical control points of Salmonella contamination and reducing the risk of foodborn Salmonella infection. Methods: The carcass surface, cloaca and crop contents of broilers, processing environments, and the surface of processing equipment and apparatus were sampled from two broiler slaughter and processing plants (FX and GZ). The routine procedure and PCR were performed for the isolation and identification of Salmonella, then enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) were used for subtyping the isolates and the NTSYS-pc 2.10 software was applied for clustering analysis. Results: The total positive rate of Salmonella in 1171 samples from both plants was 1.37% (16 positive samples). The positive rate of FX samples was lower (0.18%), and showed a significant difference from that of GZ samples (2.39%, P ＜ 0.01). None of the FX samples were found positive for genotype I Salmonella except broiler carcass surface samples. Genotype I Salmonella was detected in samples of carcass surface, cloaca and crop contents, and the surfaces of plucking fingers and eviscerators,s gloves collected from the GZ plant, and genotype III Salmonella was detected in samples from the surfaces of carcass and eviscerators,s gloves, but genotype V and II Salmonella were detected only on the surfaces of transport vehicles and plucking fingers, respectively. Conclusion: Pre-slaughter, defeathering and evisceration are the key stages of Salmonella contamination. Strengthening entry quarantine and the daily hygiene management of equipment and apparatus is helpful for reducing the risk of Salmonella contamination.

Key words: broiler, slaughter and processing, Salmonella, contamination, source tracing

References

[1] 赵瑞兰, 张培正, 李远钊. 肉鸡加工厂环境及半成品中沙门氏菌污染情况调查[J]. 中国食物与营养, 2005(9): 32-34.
[2] 李晓霞, 于爱莲, 邱玉玉, 等. 海产品中沙门氏菌的分离及其ERIC-PCR指纹分析[J]. 中国病原生物学杂志, 2010, 5(3): 164-167.
[3] 李郁, 焦新安, 魏建忠, 等. 合肥市屠宰生猪肉样沙门菌的PCR快速检测[J]. 中国卫生检验杂志, 2007, 17(11): 2018-2019.
[4] 张宏梅, 石磊, 李琳. 沙门氏菌ERIC指纹分析[J]. 云南农业大学学报, 2007, 22(4): 467-470.
[5] Li Yu, Fang Yan-Hong, Zhu Heng-Wen, et al. The analysis of ERIC-PCR genomic polymorphism of Salmonella isolated strains in pig carcass[J]. Journal of Animal and Veterinary Advances, 2011, 10(13): 1694-1698.
[6] Luiz Gustavo dos Anjos Borges, Virgínia Dalla Vechia, Gertrudes Cor??o. Characterisation and genetic diversity via REP-PCR of Escherichia coli isolates from polluted waters in southern Brazil[J]. FEMS Microbiology Ecology, 2003, 45(2): 173-180.
[7] Olsen J E, Brown D J, Madsen M, et al. Cross-contamination with Salmonella on a broiler slaughterhouse line demonstrated by use of epidemiological markers[J]. Journal of Applied Microbiology, 2003, 94(5): 826-835.
[8] Beatriz Garbelotti Matias, Paulo Sergio de Arruda Pinto, Marcus Vinicius Coutinho Cossi, et al. Salmonella spp. and hygiene indicator microorganisms in chicken carcasses obtained at different processing stages in two slaughterhouses[J]. Foodborne Pathogens and Disease, 2010, 7(3): 313-318.
[9] Sakaridis I, Soultos N, Iossifidou E, et al. Prevalence and antimicrobial resistance of Salmonella serovars from chicken carcasses in northern Greece[J]. Journal of Food Safety, 2011, 31(2): 203-210.
[10]Corry J E L, Allen V M, Hudson W R, et al. Sources of Salmonella on broiler carcasses during transportation and processing: modes of contamination and methods of control[J]. Journal of Applied Microbiology, 2002, 92: 424-432.
[11]Hinton A, Cason J A, Ingram K D.Tracking spoilage bacteria in commercial poultry processing and refrigerated storage of poultry carcasses[J]. International Journal of Food Microbiol, 2004, 91(2): 155-165.
[12]Ho CP, Huang NY, Chen BJ. A survey of microbial contamination of food contact surfaces at broiler slaughter plants in Taiwan[J].Journal of food protection, 2004, 67(12): 2809-2811.
[13]Arnold J W. Bacterial contamination on rubber picker fingers before, during, and after processing[J]. Poultry Science Association Inc. 2007, 86(12): 2671-2675.
[14]李郁, 焦新安, 魏建忠, 等. 屠宰生猪沙门氏菌分离株的血清型和药物感受性分析[J]. 中国人兽共患病学报, 2008, 24(1): 67-70.
[15]Rasschaert G, Houf K, Zutter L D. Impact of the slaughter line contamination on the presence of Salmonella on broiler carcasses[J]. Journal of Applied Microbiology, 2007, 103(2): 333-341.