Rapid Detection of Vibrio metschnikovii by Smartongue

Abstract

Abstract: The availability of Smartongue, a bionic sensor, for rapid detection of Vibrio metschnikovii was explored with the aim of proposing a rapid way to detect Vibrio metschnikovii in aquatic products. Smartongue combined with principal component analysis was used to discriminate among liquid cultures of 11 pathogenic Vibrio species to determine the availability of the combined techniques and minimum culture time. Meanwhile, optimal combinations of electrode and frequency were determined and then discrimination models for the detection of Vibrio metschnikovii was set up by combined use of Smartongue and soft independent modeling of class analogy (SIMCA) and validated. Optimal discrimination models were determined for high recognition rates to discover if combined Smartongue and SIMCA could be available for establishing a database for rapid detection of Vibrio metschnikovii. Eleven species of pathogenic Vibrio cultured in specific media for 7 h were successfully discriminated using Smartongue technique. The discrimination models based on 6 combinations of electrode and frequency showed a recognition rate of 100% for all samples. In conclusion, Smarttongue combined with SIMCA is available for rapid detection of Vibrio metschnikovii.

Key words: Smartongue, Vibrio metschnikovii, soft independent modeling of class analogy (SIMCA), rapid detection

CLC Number:

TS207.4

References

[1] 闻玉梅. 精编现代医学微生物学[M]. 上海: 复旦大学出版社, 2002: 287-293.
[2] LINDE H J, KOBUCH R, TAYASINGHE S. Vibrio metschnikovii, a Rare Cause of Wound Infection [J]. Journal of Clinical Microbiology, 2004, 42: 4909-4911.
[3] MARIA H M, LUCIA B, MARIA L B, et al. Virulence factors of Vibrio metschnikovii strains isolated from fish in Brazil [J]. Food Control, 2007, 18(6): 747-751.
[4] CETO X , GUTIERREZ J M , MORENO-BARON L, et al. Voltammetric electronic tongue in the analysis of cava wines[J]. Electroanalysis, 2011, 23 (1): 72-78.
[5] PALIT M , TUDU B , DUTTA P K. Classification of black tea taste and correlation with tea taster's mark using voltammetric electronic tongue[J]. IEEE Transactions on Instrumentation and Measurement, 2010, 59 (8): 2230-2239.
[6] LUIS G, JOSE M B, DIANA B. Monitoring of physical–chemical and microbiological changes in fresh pork meat under cold storage by means of a potentiometric electronic tongue[J]. Food Chemistry, 2011, 126: 1261-1268.
[7] TIAN S Y, DENG S P, CHEN Z X. Multifrequency Large Amplitude Pulse Voltammetry: A Novel Electrochemical Method for Electronic Tongue Sensors[J]. Sensors and Actuators B, 2007, 123: 1049-1056．
[8] SODERSTORM C, BORE H, WINQUIST F, et al. Use of an electronic tongue to analyze mold growth in liquid media[J]. International Journal of Food Microbiology, 2003, 83: 253-261.
[9] CLAIRE T, ALISA R, ANDREY L. Monitoring batch fermentations with an electronic tongue[J]. Journal of Biotechnology, 2003, 103(1): 87-91.
[10] LEGIN A, KIRSANOV D, RUDNITSKAYA A, et al. Multicomponent analysis of fermentation growth media using the electronic tongue (ET)[J]. Talanta, 2004, 64: 766-772.
[11] SODERSTORM C, UDNITSKAYA A, LEGIN A, et al. Differentiation of four Aspergillus species and one Zygosaccharomyces with two electronic tongues based on different measurement techniques[J]. Journal of Biotechnology, 2005, 119: 300-308.
[12] JORGE Y H, DIEGO P, FERNANDO B. Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin[J]. Biosensors and Bioelectronics, 2010, 25: 2470-2476.
[13] 张夏宾, 王晓萍, 田师一, 等. 新型伏安型多频脉冲电子舌及其应用[J]. 浙江大学学报（工学版）, 2008, 42(10): 1706-1709.
[14] 梁逸曾, 俞汝勤. 化学计量学[M]. 高等教育出版社, 2003, 5: 214-228.
[15] 倪永年. 化学计量学在分析化学中的应用[M]. 2004: 227-257.