Determination of Ellagic Acid in Pomegranate Seeds by RP-HPLC

Abstract

Abstract: Objective: To establish an HPLC method for the determination of ellagic acid in pomegranate seeds. Methods: The chromatographic separation was carried out on an Arcus EP-C18 column (250 mm × 4.6 mm, 5 μm) using a mobile phase composed of a mixture of methanol and 0.1% TFA at a flow rate of 1.0 mL/min by gradient elution. The detection wavelength was 254 nm. Results: The linear range was 0.004－0.064 μg for ellagic acid. The average recovery rate was 100.49%. Conclusion: This method is accurate, reproducible and applicable for quality control of ellagic acid in pomegranate seeds.

Key words: pomegranate seedsl, ellagic acid, RP-HPLC

CLC Number:

R917

[1]	HAN Fenli, HAN Fei, LI Aike, CHEN Xi, SONG Ge, FAN Liuping, YU Zhiquan. Determination of Amino Acid Contents in Cereals and Ileal Digesta by Reversed-Phase High Performance Liquid Chromatography with AccQ-Tag Pre-column Derivatization Method [J]. FOOD SCIENCE, 2018, 39(4): 165-170.
[2]	JIAO Xinxin, ZHAO Lei, WANG Kai, HU Zhuoyan. Preparation and Characterization of Sodium Alginate-Gelatin-Ellagic Acid Blended Film [J]. FOOD SCIENCE, 2018, 39(21): 260-265.
[3]	MA Jing, KANG Meiling, TIAN Zhongjing, ZHANG Yingxia, DING Chengshi, ZHANG Lihua. Preparation of Ellagic Acid Microspheres and Their Effects on Preadipocyte Growth and Adipogenic Differentiation [J]. FOOD SCIENCE, 2018, 39(17): 104-108.
[4]	LI Xin, LIU Jingling, LI Yan, GUO Wanli, WANG Dajin, LIANG Zongsuo. Structural Analysis of Proanthocyanidins from Tetrastigma hemsleyanum Diels et Gilg [J]. FOOD SCIENCE, 2018, 39(16): 247-255.
[5]	LI Rong, YU Jun, JIANG Zitao, HUANG Xianyong. Preparation of Umami Peptide by Microwave-Assisted Stepwise Enzymatic Hydrolysis of Perilla Seed Meal Protein [J]. FOOD SCIENCE, 2017, 38(20): 169-175.
[6]	RUI Hongfei, ZHANG Xiaoyu, LIU Xingquan*, WU Fenghua, WANG Zhuoran, LI Kaili. Simultaneous Determination of Free Amino Acids and Biogenic Amines in Chinese Rice Wine by RP-HPLC with Pre-Column Phenylisothiocyanate (PITC) Derivatization [J]. FOOD SCIENCE, 2016, 37(8): 159-163.
[7]	KUANG Hui, LI Liangliang, Lü Changshan, WANG Jinling,*. Determination of Organic Acids in Six Varieties of Red Raspberry Fruits Grown in Northeast China by RP-HPLC Method [J]. FOOD SCIENCE, 2016, 37(22): 126-130.
[8]	TIAN Lu, WANG Liping. Mixed Culture Fermentation and Quality Analysis of Persimmon Peel Vinegar [J]. FOOD SCIENCE, 2016, 37(19): 197-203.
[9]	YIN Peipei1, YAN Linlin1, CAO Ruoyu1, CHEN Xiaoyuan2, MA Chao1,, LIU Yujun1,. A Review of Urolithins, Gut Microflora Metabolites of Dietary Ellagic Acid [J]. FOOD SCIENCE, 2015, 36(7): 256-260.
[10]	ZHANG Lihua1, ZHANG Yuanhu2,*, SUN Lei3, ZHAO Dengchao3. Distribution of Ellagic Acid and Triterpenes in Different Organs of Pomegranate [J]. FOOD SCIENCE, 2015, 36(6): 136-139.
[11]	LI Hongwei1, WANG Kaiping1, WU Yu1, TANG Zhengjiang1, TANG Fei1, LIU Guoqing1,2,*. Determination of Organic Selenium Compounds in Selenium-Enriched Aspergillus oryzae by Pre-Column Derivatization-RP-HPLC Method [J]. FOOD SCIENCE, 2015, 36(22): 137-141.
[12]	BAI Juhong, TANG Shanhu*, LI Sining, WANG Liu, LU Fuqing, YAN Liguo. Effects of Three Strains of Lactic Acid Bacteria and Their Combinations on the Peptide Changes in Fermented Yak Meat Sausage [J]. FOOD SCIENCE, 2015, 36(17): 185-190.
[13]	WEI Zheng1, ZHAO Yajiao1, HUANG Yu2, ZHANG Yali1, LU Jiang1,*. Optimization of Ultrasound-Assisted Extraction of Ellagic Acid and Total Phenols from Muscadine (Vitis rotundifolia) by Response Surface Methodology [J]. FOOD SCIENCE, 2015, 36(12): 29-35.
[14]	WANG Jinling1, LI Liangliang1, Lü Changshan2. Determination of Ellagic Acid from Six Red Raspberry Cultivars Grown in Northeast China Using RP-HPLC [J]. FOOD SCIENCE, 2015, 36(12): 93-96.
[15]	HU Jia-wei1, GAO Rong2, CAO Min-jie1, CAI Qiu-feng1, ZHANG Ling-jing1, SU Wen-jin1, LIU Guang-ming1,*. Establishment and Application of Dansyl Chloride Precolumn Derivatization RP-HPLC for Determination of Histamine in Aquatic Products [J]. FOOD SCIENCE, 2014, 35(8): 283-288.

Determination of Ellagic Acid in Pomegranate Seeds by RP-HPLC

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments