Optimization of Ultrasound-Assisted Extraction of Ellagic Acid and Total Phenols from Muscadine (Vitis rotundifolia) by Response Surface Methodology

doi:10.7506/spkx1002-6630-201512006

Abstract

Abstract:

The extraction conditions of ellagic acid and total phenols from the skin and seeds of the Noble muscadine
using acetone/water/hydrochloric acid (70:29:1, V/V) by ultrasonic-assisted extraction (UAE) were optimized employing a
combination of single-factor experiments and response surface methodology through mathematical modeling. The analysis
of two-factor interactions was carried out. The results indicated the ratio of solid to liquid, extraction time and ultrasonic
power to be the most significant factors affecting the extraction efficiencies of ellagic acid and total phenols. The optimum
extraction conditions for ellagic acid and total phenols from muscadine skin were determined as follows: ratio of solid to
liquid, 1:30; extraction time, 28 min; and ultrasonic power, 616 W. The predicted contents of ellagic acid and total phenols
under these conditions were 616.21 μg/g and 15.06 mg/g (using gallic acid equivalent, fresh weight), respectively, agreeing
with the experimental values. In conclusion, UAE can be applied to the extraction of ellagic acid.

Key words: muscadine, ellagic acid, ultrasonic-assisted extraction, response surface methodology

CLC Number:

WEI Zheng1, ZHAO Yajiao1, HUANG Yu2, ZHANG Yali1, LU Jiang1,*. Optimization of Ultrasound-Assisted Extraction of Ellagic Acid and Total Phenols from Muscadine (Vitis rotundifolia) by Response Surface Methodology[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201512006.

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Optimization of Ultrasound-Assisted Extraction of Ellagic Acid and Total Phenols from Muscadine (Vitis rotundifolia) by Response Surface Methodology

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