Determination of Avemectin Residues in 10 Foods by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

Abstract

Abstract: A reliable analytical method to determine 6 avermectin residues in 10 foods was developed using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Samples were extracted with acetonitrile, evaporated in a rotary evaporator until dryness, re-dissolved in a mixture of acetonitrile and water (1:9, V/V), cleaned up on a PEP-C18 cartridge, blown under nitrogen gas until dryness, and then re-dissolved in acetonitrile prior to UPLC-MS/MS analysis by positive ion electrospray ionization (ESI+) in multiple reaction monitoring (MRM) mode. The calibration curves of 6 avermectins were linear in the concentration of 5－250μg/kg (R2＞ 0.99). The average recovery rates at three spiked levels were 60%－99% with relative standard deviations (RSDs) of 0.2%－8.9%. The quantification limits for 6 avermectins (RSN≥10) were slightly lower than 5.0 μg/kg. This analytical method could meet the requirements for trace analysis.

Key words: abamectins, solid phase extraction, ultra-high high performance liquid chromatography-tandem mass spectrometry

CLC Number:

TS207

References

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