Optimization of Ultrasound-Assisted Extraction Process for Oleuropein from Olea europaea Leaves by Response Surface Analysis

Abstract

Abstract: Objective: To find optimal process conditions for the ultrasound-assisted extraction of oleuropein from Olea europaea leaves. Methods: The effects of methanol concentration, solid-to-solvent ratio and extraction time on oleuropein yield were investigated by one-factor-at-a-time design. A three-factor, three-level Box-Behnken experimental design was employed to build a second-order polynomial regression equation. The obtained regression equation was analyzed by response surface analysis to optimize the three process conditions. Results: The optimal process conditions were as follows: methanol concentration of 93.5%, solid-to-solvent ratio of 1:25 (g/mL) and an extraction time of 26 min. Conclusion: Under these conditions, the oleuropein yield from Olea europaea leaves was up to 7.83%.

Key words: Olea europaea leaves, oleuropein, ultrasound-assisted extraction, response surface methodology

CLC Number:

TS201.1

References

参考文献：
[1] 林鹏, 王洁雪, 李晓鲁, 等. 油橄榄叶中裂环烯醚萜类化合物的研究进展[J]. 西南民族大学学报, 2008, 34(5): 999-1002.
[2] 高彩霞, 王成章, 陈文英, 等. 油橄榄叶中多酚和黄酮的含量分析[J]. 生物质化学工程, 2006, 40(4): 4-6.
[3] 郝婷, 崔建玲, 赵桂琴, 等. 橄榄苦苷提取分离和药理活性的研究进展[J]. 承德医学院学报, 2010, 27(1): 69-72.
[4] 陈千良, 孙文基. 裂环烯醚菇类化合物研究进展[J]. 国外医药.植物药分册, 2003, 18(2): 58-62.
[5] LEONARDIS A D, ARETINI A, ALFANO G, et al. Isolation of a Hydroxytyrosol-rich Extract from Olive Leaves(Olea Europaea L.) and Evaluation of its Antioxidant Propertiesand Bioactivity[J]. Eur Food Res Technol, 2008, 226: 653-659.
[6] RIDA F, SAMER B, SERINE E, et al. The Antioxidant and Anti-proliferative Activity of the Lebanese Olea Europaea Extract[J].Plant Foods Hum Nutr, 2010, 10(7): 9-12.
[7] VALENTINA R G, ROCIO P, ANGEL C. The Effect of Tyrosol, Hydroxytyrosol and Oleuropein on the Non-enzymatic Lipid Peroxidation of Rat Liver Microsomes[J]. Molecular and Cellular Biochemistry, 2001, 217: 35-41.
[8] 王成章, 高彩霞, 叶建中等. 引种阿斯油橄榄叶中橄榄苦苷提取分离及结构鉴定[J]. 林产化学与工业, 2009, 29(3): 53-57.
[9] 何志勇, 夏文水. 橄榄果实中酚类化合物的分析研究[J]. 安徽农业科学, 2008 , 36(26): 406-407.　
[10] RYAN D, LAWRENCE H, PRENZLER P, et al. Recovery of Phenolic Compounds from Olea Europaea[J]. Analytica Chimica Acta, 2001, 445: 67–77.
[11] KIRITSAKIS K, KONTOMINAS M G, KONTOGIORIS C, et al. Composition and Antioxidant Activity of Olive Leaf Extracts from Greek Olive Cultivars[J]. J Am Oil Chem Soc, 2010,87: 369-376.
[12] 林健, 陈锋, 李春来. 橄榄总黄酮的超声提取工艺研究[J]. 海峡药学, 2007, 19(11): 60-62.　
[13] 李张伟, 刘宇辉, 张珠珠. 橄榄黄酮的提取及其抗氧化作用的研究[J]. 广东化工, 2007, 12(34): 37-41.
[14] 任丹丹, 陈谷. 响应面法优化黄秋葵多糖超声提取工艺[J]. 食品科学, 2011, 32(8): 143-146.
[15] 党建章, 许柏球, 徐华顺, 等. 高效液相色谱法测定橄榄叶提取物中橄榄苦普的含量[J]. 中国药学杂志, 2006, 41(9): 711-712