Detection of Listeria monocytogenes by IC-PCR and Direct PCR

Abstract

Abstract: Immuno-capture PCR (IC-PCR), established by integrating immunological and molecular biological techniques was compared with traditional direct PCR. The sensitivity (104 CFU/mL) of IC-PCR for pure Listeria monocytogenes culture suspensions indicated a 10-fold increase compared with direct PCR (104 CFU/mL). The limit of detection of IC-PCR was 5 × 101 bacteria/PCR reaction system (i.e. 104 CFU/mL), which was 100 times higher than that of direct PCR (5 × 103 bacteria/PCR reaction system, 106 CFU/mL). IC-PCR had high specificity and anti-interference capability for detecting Listeria monocytogenes. Moreover, no cross-reactivity with 18 common foodborne pathogens was observed. Therefore, IC-PCR is a rapid, economical, sensitive and specific so that it can be suitable for rapid diction of Listeria monocytogenes in food safety supervisory agencies and food enterprises.

Key words: Listeria monocytogenes, IC-PCR, direct PCR

CLC Number:

TS207.4

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