Immunological Characterization and Elimination of Listeria Phage in vivo

Abstract

Abstract: To explore the safety of Listeria phage as a biological antimicrobic agent, its elimination and immune responses to it in BALB/c mice were analyzed. Mice were immunized by oral single-dose or triple-dose (once daily on 3 consecutive days) administration of 1×108 PFU of phage LipG2-5 and the number of phages in mouse feces sampled at different time points following administration was examined and body weight was measured. Phage treatment groups showed no significant difference in body weight from control group. Meanwhile, serum and intestinal mucosa were collected for analysis of the production of specific antibodies. As a result, the number of phages declined over time after administration and was no longer detectable in mouse feces from 7 until 35 d after administration. ELISA analysis indicated that oral administration of phages induced a low level of specific antibodies in the serum of mice without significant differences compared with control group. Similarly, the measured level of mucosal IgA 35 d after administration was not high. From these results, we conclude that Listeria phage can be directly excreted through the digestive tract without being retained in the body and inducing antibodies.

Key words: Listeria monocytogenes, phage, antibody

CLC Number:

Q939.48

References

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