Intraspecific Genetic Diversity of Lactic Acid Bacteria from Grape Wine

Abstract

Abstract: Lactic acid bacteria isolated from spontaneously Malolactic fermented grape swine were identified by biochemical identification and 16S rRNA sequencing. The results showed that the strains were Oenococcus oeni, Lactobacillus mali, Lactobacillus plantarum and Lactobacillus brevis. Intraspecific genetic diversity of these strains was further analyzed by single-enzyme amplified fragment length polymorphism (SE-AFLP) and phylogenetic tree was constructed by the method of pair group method with arithmetic Mean (UPGMA). The results showed that SE-AFLP could identify lactic acid bacteria to strain level in lactic acid bacteria classification.

Key words: grape wine, lactic acid bacteria, single-enzyme amplified fragment length polymorphism (SE-AFLP), intraspecific identification

CLC Number:

TS261.1

References

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