Screening and Mutation Breeding of a High-Yield γ-Aminobuyric Acid(GABA) Mould Strain

Abstract

Abstract: In this study, different species of moulds were analyzed for effectiveness in fermenting cooked soybeans suspended in water (3:5, m/V) to produce γ-aminobuyric acid (GABA). As a result, Aspergillus oryzae 3.800 was found to have the highest GABA producing ability. The strain was mutagenized by UV irradiation for 2 min. For secondary screening, the resulting mutant strains were cultured initially in PDA medium containing 1 g/100 mL L-glutamic acid and then in soybean suspension. A stable high-yield mutant named as 3.800-4 was obtained. The GABA yield of the mutant was 4.491 g/L during culture in PDA medium containing 1 g/100 mL L-glutamic acid and 0.874 g/L during culture in soybean suspension, which was increased by 23.58% and 29.67%, respectively, compared with the original strain.

Key words: γ-aminobuyric acid, mould, screening, UV-induced mutagenesis, high-yield mutant strain

CLC Number:

理论与应用研究学术论文

Xin BIAN. Screening and Mutation Breeding of a High-Yield γ-Aminobuyric Acid(GABA) Mould Strain[J]. FOOD SCIENCE, 2012, 33(21): 213-216.

References

[1] 陈恒文,林健.桑叶中γ-氨基丁酸的研究概述[J].食品工业科技,2008,(8):298-304
[2] Maria del Carmen Garcia,Edda Adler-Graschinsky,Stella Maris Celuch. Role of CGRP and GABA in the hypotensive effect of intrathecally administered anandamide to anesthetized rats [J]. European Journal of Pharmacology,2006 (532): 88-98
[3] Dorothy M Jones-Davis, Robert L Macdonald. GABAA receptor function and pharmacology in epilepsy and status epilepticus [J]. Current opinion in pharmacology.2003,3 (1):12-18
[4] 于霞,史正刚,姬可平. GABA受体在儿童失神性癫痫发病机制中的作用[J]. 中国优生与遗传杂志,2004，12(5):10-11
[5] 李瑞芝,郭建友,李昌煜等.焦虑症γ-氨基丁酸受体机制与药物干预研究进展[J]. 中国药理学通报,2010,26(9):
[6] 叶惟怜.γ-氨基丁酸的发现史[J].生理科学进展,1986,17(2):187-189
[7] S.Yokoyama,J.I.Hiramatsu,K.Hayakawa.Production of γ-Aminobutyric Acid from Alcohol Distillery Lees by Lactobacillus brevis IFO-12005[J].Journal of Bioscience and Bioengineering,2002,93(1):95-97.
[8] Noriko Komatsuzaki,Jun Shima,Shinichi Kawamoto,Hiroh Momose,Toshinori Kimura.Production of γ-aminobutyric acid(GABA) by Lactobacillus paracasei isolated from traditional fermented foods[J].Food Microbiology 2005,22:497–504
[9] 李海星,江英英.产γ-氨基丁酸乳酸菌的筛选及初步鉴定[J].天然产物研究与开发,2007,19(3):455-510
[10] 蒋冬花,后加衡,黄大年.酸菜中高产γ-氨基丁酸乳酸菌的筛选[J].微生物学杂志,2007,27(1):35-39
[11] 李远宏,吕凤霞,邹晓葵.新鲜奶中产γ-氨基丁酸乳酸菌株的筛选与鉴定[J].食品科学,2010,31 (15):198-202
[12] Y.C.Su,J.J.Wang,T.T.Lin. Production of the secondary metabolites γ-aminobutyric acid and monacolin K by Monascus[J].Ind Microbiol Biotechnol,2003(1):41-46.
[13] 蒋冬花,李杰,后家衡.水果表面高产γ-氨基丁酸酵母菌菌株的筛选、鉴定和发酵条件优化[J].浙江农业学报,2008,20(6):396-401
[14] 黄丽华,胡超,左斌等.高产γ-氨基丁酸酵母菌株的亚硝基胍诱变选育[J].现代生物医学展,2010,10(6):1106-1109
[15] Kitaoka S , Nakano Y . Colorimetric determination of ω- amino acids [J].The Journal of Biochemistry ,1969, 66(1): 87-94